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On day 2, this is what the zebrafish eggs look like under the microscope at 30x magnification. 1
On day 1, four tanks of zebrafish were setup each with different letters corresponding to the different types. Each group of students were assigned a certain genetic cross of two zebrafish. The crosses included A x D, B x C and C x D. 2
Juniors Oliver Baron, Raqib Hossain, and Don Bui, all in a group, received the genetic cross B x C and began to retrieve the fish for their specific genetic cross.3
For some groups, retrieving the fish was fairly simple. Other groups struggled to retrieve their fish. 4
Once the fish were retrieved, a label was put on their containers identifying which genetic cross they were. Then the containers were stacked up as the groups awaited further instruction.5
The groups then made observations about their two fish, whether they had different sizes, colors and behaviors.6
Ms. Mills demonstrates to the class were the fish will go after class and the mechanism used to induce mating between the two fish. 7
On day 3, students had to use small pipettes to remove the the dead eggs and if hatched already, the empty cases. If the the dead eggs and empty cases were not removed, as they decompose, they could release toxins that may kill other eggs and hatching larvae. 8
On day 4, majority of the larvae are hatched and could be seen with the naked eye. Many students found that they had pigmented and nacre (non-pigmented) zebrafish larvae in there petri dishes. 9
On the final day all the larvae have hatched and body parts can be distinguished in the microscope.10
With the help of Ms. Sloe, students took one larvae zebrafish and added it to a microscope slide. A muscle relaxant was then added to the larvae to stop them from moving.11
Junior Nika Lilley observes a zebrafish larvae at 400x magnification. At that magnification the zebrafish's heart, and blood vessels can be seen. 12
Zebra Fish
During the school week from March 7 - 11, Darcy Sloe's biology classes had the opportunity to work with zebrafish in a week long genetics lab known as Project BioEyes. Project BioEyes is an educational program that has provided classroom-based learning of genetics in several states. Thanks to the Blair PTSA and the BioEyes staff, Sloe and Ms.Mills received training in December and brought the zebrafish project to Blair. In the future Sloe hopes to continue the use of zebrafish as model to help students.
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On day 2, this is what the zebrafish eggs look like under the microscope at 30x magnification.
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